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For example, individuals dealing with dehydration, fatigue, or immune system issues may benefit from IV hydration therapy combined with electrolytes and B vitamins

GST-OLFM4 protein was expressed in BL21 (DE3) by 0.5 M IPTG inducing overnight, then harvest cells and resuspend in phosphate-buffered saline (PBS) (0.1% Triton100, Protease Inhibitor Cocktail, 1 mM PMSF), after lyzed by sonication, proteins were purified by glutathione Sepharose 4B (GE healthcare)

J Biol Chem 284: 75537560 MorganB,SobottaMC,DickTP (2011) Measuring E(GSH) and H(2)O(2) with roGFP2based redox probes

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